Abstract

Bootstrapping was used to examine the effect of sampling error and measurement error and its correlation on fixedbin genotype probabilities. Bootstrap confidence intervals (CIs) were made relative to the point estimate using the log of the inverse of the probabilities. From databases of 200–250 genotypes, sampling error alone yielded median relative 95% CIs of from one order of magnitude out of five for one locus to one out of ten for four loci. Measurement error of the test genotype fragments increased the latter to about one order of magnitude out of eight. Database measurement error and its correlation had only a slight effect on multi-locus probability uncertainty. Together, these uncertainties are several orders of magnitude greater than error due to population substructuring of a race by its major component ethnic groups.

Issue Section:
Research Papers
Keywords:
forensic science, DNA typing, sampling error, measurement error, restriction fragment length polymorphism, variable number of tandem repeats, genotype, population genetics

References

1.
Weir
 
B
.
Population genetics in the forensic DNA debate
.
Proc Nat Acad Sci USA
1992
;
89
:
11654
-
9
.
Google Scholar
Crossref
Search ADS
PubMed
 
2.
Evett
 
I W
,
Werret
 
D J
,
Gill
 
P
,
Buckleton
 
J S
.
DNA fingerprinting on trial
.
Nature
 0028-0836
1989
;
340
:435.
3.
Shapiro
 
M M
.
Imprints on DNA fingerprints
.
Nature
 0028-0836
1991
;
353
:
121
-
2
.
Google Scholar
Crossref
Search ADS
PubMed
 
4.
Evett
 
I W
.
Trivial error
.
Nature
 0028-0836
1991
;
354
:114.
5.
Berry
 
D A
,
Evett
 
I W
,
Pinchin
 
R
.
Statistical inference in crime investigations using deoxyribonucleic acid profiling
.
Appl Stat
1992
;
41
(
3
):
499
-
531
.
Google Scholar
Crossref
Search ADS
 
6.
Devlin
 
B
,
Risch
 
N
,
Roeder
 
K
.
Forensic inference from DNA fingerprints
.
J Am Stat Assn
1992
;
87
:
337
-
50
.
Google Scholar
Crossref
Search ADS
 
7.
Chakraborty
 
R
.
Sample size requirements for addressing the population genetic issues of forensic use of DNA typing
.
Hum Biol
1992
;
64
(
2
):
141
-
59
.
Google Scholar
PubMed
 
8.
Evett
 
I W
,
Gill
 
P
.
A discussion of the robustness of methods for assessing the evidential value of DNA single locus profiles in crime investigations
.
Theor Appl Electrophoresis
1991
;
12
:
226
-
30
.
Google Scholar
Crossref
Search ADS
 
9.
National Research Council Committee on DNA Forensic Science
.
The evaluation of forensic DNA evidence
.
Washington (DC)
,
National Academy Press
,
1996
(prepublication copy); 4,1-5,41.
PubMed
 
10.
National Research Council Committee on DNA Technology in Forensic Science
.
DNA technology in forensic science
.
Washington (DC)
,
National Academy Press
,
1992
;
74
-
96
.
PubMed
 
11.
Chakraborty
 
R
.
Evaluation of standard error and confidence interval of estimated multilocus genotype probabilities, and their implications in DNA forensics
.
Am J Hum Genet
1993
;
52
:
60
-
70
.
Google Scholar
PubMed
 
12.
Goodman
 
L A
.
On the exact variances of products
.
J Am Stat Assn
1960
;
55
:
708
-
13
.
Google Scholar
Crossref
Search ADS
 
13.
Goodman
 
L A
.
On the exact product of k random variables
.
J Am Stat Assn
1962
;
57
:
54
-
60
.
14.
Budowle
 
B
.
A protocol for RFLP analysis of forensic biospecimens
.
Proceedings of the International Symposium on the Forensic Aspects of DNA Analysis
;
1989
;
Quantico (VA). Washington (DC)
,
US Government Printing Office
,
1989
;
57
-
62
.
15.
Baechtel
 
F S
.
The extraction, purification and quantitation of DNA
.
Proceedings of the International Symposium on the Forensic Aspects of DNA Analysis
;
1989
;
Quantico (VA). Washington (DC)
,
US Government Printing Office
,
1989
;
25
-
8
.
16.
Budowle
 
B
,
Baechtel
 
F S
.
Modifications to improve the effectiveness of restriction fragment length polymorphism typing
.
Appl Theor Electrophoresis
1990
;
1
:
181
-
7
.
17.
Monson
 
K L
,
Budowle
 
B
.
A system for semi-automated analysis of DNA autoradiograms
.
Proceedings of the International Symposium on the Forensic Aspects of DNA Analysis
;
1989
;
Quantico (VA), Washington (DC)
,
US Government Printing Office
,
1989
;
127
-
32
.
18.
Efron
 
B
.
The jackknife, the bootstrap and other resampling plans
. Regional Conference Series in Applied Mathematics, No. 38;
Philadelphia
,
Society for Industrial and Applied Mathematics
;
1982
.
Google Scholar
Crossref
Search ADS
 
19.
Devlin
 
B
,
Risch
 
N
,
Roeder
 
K
.
No excess of homozygosity at loci used for DNA fingerprinting
.
Science
 1095-9203
1990
;
249
:
1416
-
20
.
Google Scholar
Crossref
Search ADS
PubMed
 
20.
Chakraborty
 
R
,
DeAndrade
 
M
,
Daiger
 
S P
,
Budowle
 
B
.
Apparent heterozygote deficiencies observed in DNA typing data and their implications in forensic applications
.
Ann Hum Genet
1992
;
56
:
45
-
57
.
Google Scholar
Crossref
Search ADS
PubMed
 
21.
Budowle
 
B
,
Monson
 
K L
.
A statistical approach for VNTR analysis
.
Proceedings of an International Symposium on the Forensic Aspects of DNA Analysis
;
1989
;
Quantico (VA); Washington (DC)
,
US Government Printing Office
,
1989
;
121
-
6
.
22.
Hartmann
 
J M
,
Houlihan
 
B T
,
Keister
 
R S
,
Buse
 
E L
.
The effect of ethnic and racial population substructuring on the estimation of multi-locus fixed-bin VNTR RFLP genotype probabilities
.
J Forensic Sci
1997
;
42
(
2
):
231
-
9
.
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