Abstract

Age determinations of adult individuals are of great importance within the forensic sciences. Presently, age changes in the teeth provide good means for this. The best method up to now has been “Gustafson's method” with modifications. Over the last 10 years a new chemical method, based on the racemization of aspartic acid in enamel and dentine, has been developed. Enamel and dentine belong to the very few tissues that do not have metabolic turn-over after formation. Both reliability and validity have been shown to be high, and this method therefore seems to be the best choice for the future. However, most studies to date have been using expensive and special instruments (gas chromatographs or amino acid analyzers) for these determinations. This study was performed to explore if the same determinations can be carried out with ordinary HPLC technique. It was shown that there is a good correlation between the ratio of D- and L-forms of the aspartic acid and age of the tooth. The correlation coefficient was 0.97, which is close to those presented with the other techniques, where r has been 0.95 – 0.99. The prediction of an individual are can be made with a 95% confidence interval of about ± 12 years.

Issue Section:
Research Papers
Keywords:
odontology, age determination by teeth, aspartic acid, high pressure liquid chromatography

References

1.
Gustafson
,
G.
, “
Age Determinations on Teeth
,”
Journal of American Dental Association
, Vol.
41
, No.
1
,
07
1950
, pp.
45
-
54
.
Google Scholar
Crossref
Search ADS
 
2.
Dalitz
,
D. G.
, “
Age Determination of Adult Human Remains by Teeth Examination
,”
Journal of Forensic Science Society
, Vol.
3
, No.
1
,
09
1962
, pp.
11
-
21
.
Google Scholar
Crossref
Search ADS
 
3.
Johanson
,
G.
, “
Age Determination from Human Teeth
,”
Odontologisk Revy
 0029-8441, Vol.
22
, Special Issue,
1971
, pp.
1
-
126
.
Google Scholar
PubMed
 
4.
Maples
,
W. R.
 and
Rice
,
P. M.
, “
Some Difficulties in the Gustafson Dental Age Estimations
,”
Journal of Forensic Sciences
 0022-1198, Vol.
24
, No.
1
,
01
1979
, pp.
168
-
172
.
Google Scholar
Crossref
Search ADS
PubMed
 
5.
Metzger
,
Z.
,
Buchner
,
A.
, and
Gorsky
,
M.
, “
Gustafson's Method for Age Determination from Teeth—A Modification for the Use of Dentists in Identification Teams
,”
Journal of Forensic Sciences
 0022-1198, Vol.
25
, No.
4
,
10
1980
, pp.
742
-
749
.
Google Scholar
Crossref
Search ADS
PubMed
 
6.
Chomette
,
G.
,
Auriol
,
M.
,
Koulibaly
,
M.
,
Bellefqih
,
S.
,
Guilbert
,
F.
, and
Vaillant
,
J. M.
, “
Approche de détermination de l'âge sur critères morphologiques dentaires obtenus en microradiographie, stéréomicroscopie et microscopie à balayage
,”
Revue de Stomatologie et de Chirurgie Maxillo-Faciale
, Vol.
87
, No.
1
,
1986
, pp.
30
-
33
.
Google Scholar
PubMed
 
7.
Kashyap
,
V. K.
 and
Koteswara Rao
,
N. R.
, “
A Modified Gustafson Method of Age Estimation from Teeth
,”
Forensic Science International
 0379-0738, Vol.
47
, No.
3
,
10
1990
, pp.
237
-
247
.
Google Scholar
Crossref
Search ADS
PubMed
 
8.
Nkhumeleni
,
F. S.
,
Raubenheimer
,
E. J.
, and
Monteith
,
B. D.
, “
Gustafson's Method for Age Determination, Revised
,”
Journal of Forensic Odonto-Stomatology
 0258-414X, Vol.
7
, No.
1
,
06
1989
, pp.
13
-
16
.
Google Scholar
PubMed
 
9.
Xu
,
X. H.
,
Philipsen
,
H. P.
,
Jablonski
,
N. G.
,
Weatherhead
,
B.
,
Pang
,
K. M.
, and
Zhu
,
J. Z.
, “
Preliminary Report on a New Method of Human Age Estimation from Single Adult Teeth
,”
Forensic Science International
 0379-0738, Vol.
51
, No.
2
,
11
1991
, pp.
281
-
288
.
Google Scholar
PubMed
 
10.
Lamendin
,
H.
,
Baccinom
,
E.
,
Humbert
,
J. F.
,
Tavernier
,
J. C.
,
Nossintchouk
,
R. M.
, and
Zerilli
,
A.
, “
A Simple Technique for Age Estimation in Adult Corpses: the Two Criteria Dental Method
,”
Journal of Forensic Sciences
 0022-1198, Vol.
37
, No.
5
,
09
1992
, pp.
1373
-
1379
.
Google Scholar
Crossref
Search ADS
PubMed
 
11.
Solheim
,
T.
, “
A New Method for Dental Age Estimation in Adults
,”
Forensic Science International
 0379-0738, Vol.
59
, No.
2
,
05
1993
, pp.
137
-
147
.
Google Scholar
Crossref
Search ADS
PubMed
 
12.
Bada
,
J. L.
,
Luyendyk
,
B. P.
, and
Maynard
,
J. B.
, “
Marine Sediments: Dating by Racemization of Amino Acids
,”
Science
 1095-9203, Vol.
170
, No.
959
,
11
1970
, pp.
730
-
732
.
Google Scholar
PubMed
 
13.
Masters
,
P. M.
 and
Bada
,
J. L.
, “
Amino Acid Racemization Dating of Bone and Shell
,”
Advances in Chemistry Series
 0065-2393, Vol.
171
, No.
8
,
08
1978
, pp.
117
-
138
.
14.
Helfman
,
P. M.
 and
Bada
,
J. L.
, “
Aspartic Acid Racemization in Tooth Enamel from Living Humans
,”
Proceedings of the National Academy of Sciences of the United States of America
 0027-8424, Vol.
72
, No.
8
,
08
1975
, pp.
2891
-
2894
.
Google Scholar
Crossref
Search ADS
PubMed
 
15.
Helfman
,
P. M.
 and
Bada
,
J. L.
, “
Aspartic Acid Razemization in Dentine as a Measure of Ageing
,”
Nature
 0028-0836, Vol.
262
, No.
5566
,
07
1976
, pp.
279
-
281
.
Google Scholar
Crossref
Search ADS
PubMed
 
16.
Ogino
,
T.
,
Ogino
,
H.
, and
Nagy
,
B.
, “
Application of Aspartic Acid Racemization to Forensic Odontology: Post Mortem Designation of Age at Death
,”
Forensic Science International
 0379-0738, Vol.
29
, No.
4
,
12
1985
, pp.
259
-
267
.
Google Scholar
PubMed
 
17.
Masters
,
P. M.
, “
Age at Death Determinations for Autopsied Remains Based on Aspartic Acid Racemization in Tooth Dentine: Importance of Postmortem Conditions
,”
Forensic Science International
 0379-0738, Vol.
32
, No.
3
,
11
1986
, pp.
179
-
184
.
Google Scholar
Crossref
Search ADS
PubMed
 
18.
Ogino
,
T.
 and
Ogino
,
H.
, “
Application to Forensic Odontology of Aspartic Acid Racemization in Unerupted and Supernumerary Teeth
,”
Journal of Dental Research
 0022-0345, Vol.
67
, No.
10
,
10
1988
, pp.
1319
-
1322
.
Google Scholar
Crossref
Search ADS
PubMed
 
19.
Ohtani
,
S.
,
Kato
,
S.
,
Sugeno
,
H.
,
Sugimoto
,
H.
,
Marumo
,
T.
,
Yamazaki
,
M.
, and
Yamamoto
,
K.
, “
A Study on the Use of the Amino-Acid Racemization Method to Estimate the Ages of Unidentified Cadavers from Their Teeth
,”
Bulletin of Kanagawa Dental College
, Vol.
16
, No.
1
,
03
1988
, pp.
11
-
21
.
20.
Ohtani
,
S.
,
Sugeno
,
H.
,
Marumo
,
T.
, and
Yamamoto
,
K.
, “
Two Cases of Age Estimation from Teeth of Burned Body Using Amino Acid Racemization
,”
Nippon Hoigaku Zasshi
 0047-1887, Vol.
43
, No.
3
,
06
1989
, pp.
191
-
197
.
Google Scholar
PubMed
 
21.
Ohtani
,
S.
 and
Yamamoto
,
K.
, “
Age Estimation Using the Racemization of Aspartic Acid on Human Dentine
,”
Nippon Hoigaku Zasshi
 0047-1887, Vol.
41
, No.
3
,
06
1987
, pp.
181
-
190
.
Google Scholar
PubMed
 
22.
Ohtani
,
S.
 and
Yamamoto
,
K.
, “
Estimating Age Through the Amino Acid Racemization of Acid-Soluble Dentinal Peptides
,”
Nippon Hoigaku Zasshi
 0047-1887, Vol.
44
, No.
4
,
08
1990
, pp.
342
-
345
.
Google Scholar
PubMed
 
23.
Ohtani
,
S.
 and
Yamamoto
,
K.
, “
Racemization Velocity of Aspartic Acid in Dentine
,”
Nippon Hoigaku Zasshi
 0047-1887, Vol.
44
, No.
4
,
08
1990
, pp.
346
-
351
.
Google Scholar
PubMed
 
24.
Ohtani
,
S.
 and
Yamamoto
,
K.
, “
Age Estimation Using the Racemization of Amino Acid in Human Dentine
,”
Journal of Forensic Sciences
 0022-1198, Vol.
36
, No.
3
,
05
1991
, pp.
792
-
800
.
Google Scholar
Crossref
Search ADS
PubMed
 
25.
Ohtani
,
S.
 and
Yamamoto
,
K.
, “
Age Estimation by Amino Acid Racemization in Teeth—A Comparison of Aspartic Acid with Glutamic Acid and Alanine As Indicators
,”
Nippon Hoigaku Zasshi
 0047-1887, Vol.
45
, No.
2
,
04
1991
, pp.
119
-
123
.
Google Scholar
PubMed
 
26.
Ohtani
,
S.
 and
Yamamoto
,
K.
, “
Estimation of Ages from Racemization of an Amino Acid in Teeth-Assessment of Errors Under Various Experimental Conditions
,”
Nippon Hoigaku Zasshi
 0047-1887, Vol.
45
, No.
2
,
04
1991
, pp.
124
-
127
.
Google Scholar
PubMed
 
27.
Ohtani
,
S.
 and
Yamamoto
,
K.
, “
Estimation of Age from a Tooth by Means of Racemization of an Amino Acid, Especially Aspartic Acid—Comparison of Enamel and Dentine
,”
Journal of Forensic Sciences
 0022-1198, Vol.
37
, No.
4
,
07
1992
, pp.
1061
-
1067
.
Google Scholar
Crossref
Search ADS
PubMed
 
28.
Ritz
,
S.
,
Schütz
,
H. W.
, and
Schwarzer
,
B.
, “
The Extent of Aspartic Acid Racemization in Dentine: A Possible Method for a More Accurate Determination of Age at Death?
,”
Zeitschrift für Rechtsmedizin
, Vol.
103
, No.
6
,
06
1990
, pp.
457
-
462
.
Google Scholar
Crossref
Search ADS
 
29.
Ritz
,
S.
,
Schütz
,
H. W.
, and
Peper
,
C.
, “
Postmortem Estimation of Age at Death Based on Aspartic Acid Racemization in Dentine: Its Applicability for Root Dentine
,”
International Journal for Legal Medicine
, Vol.
105
, No.
5
,
03
1993
, pp.
289
-
293
.
Google Scholar
Crossref
Search ADS
 
30.
Aswad
,
D. W.
, “
Determination of D-and L-Aspartate in Amino Acid Mixtures by High-Performance Liquid Chromatography After Derivatization with a Chiral Adduct of ophthaldialdehyde
,”
Analytical Biochemistry
 0003-2697, Vol.
137
, No.
2
,
03
1984
, pp.
405
-
409
.
Google Scholar
Crossref
Search ADS
PubMed
 
31.
Haavikko
,
K.
, “
The Formation and the Alveolar and Clinical Eruption of the Permanent Teeth. An Orthopantomographic Study
,”
Suomen Hammaslääkariseuran Toimituksia
, Vol.
66
, Special Issue
1970
, pp.
103
-
170
.
32.
van den Oetelaar
,
P. J. M.
,
van Beckhoven
,
R. C. M.
, and
Hoenders
,
H. J.
, “
Analysis of Aspartic Acid Racemization. Evaluation of a Chiral Capillary Gas Chromatographic and a Diastereomeric High-Performance Liquid Chromatogaphic Method
,”
Journal of Chromatography
 0021-9673, Vol.
388
, No.
2
,
02
1987
, pp.
441
-
447
.
Google Scholar
PubMed
 
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