Abstract
A routine method is described for obtaining reproducible haptoglobin patterns from bloodstains by discontinuous polyacrylamide gel electrophoresis. By employing a stacking gel, proteins from bloodstain extracts are concentrated into narrow zones, before entering the resolving gel. This effect yields highly resolved haptoglobin patterns. Therefore, laboratories without the specialized equipment and expertise for polyacrylamide gradient gel preparation can still obtain highly resolved haptoglobin patterns from bloodstains.
Issue Section:
Technical Notes
References
1.
Smithies
, O.
and Walker
, N.
, “Notation of Serum Protein Groups and the Genes
,” Nature
0028-0836, Vol. 178
, 1956
, pp. 694
-695
.2.
Smithies
, O.
, Connell
, G.
, and Dixon
, G.
, “Inheritance of Haptoglobin Subtypes
,” American Journal of Human Genetics
0002-9297, Vol. 14
, 1962
, pp. 14
-21
.3.
Stolorow
, M. D.
and Wraxall
, B. G. D.
, “An Efficient Method to Eliminate Streaking in the Electrophoretic Analysis of Haptoglobin in Bloodstains
,” Journal of Forensic Sciences
0022-1198, Vol. 24
, No. 4
, 10
1979
, pp. 856
-863
.4.
Culliford
, B.
, Biology Methods Manual
, Metropolitan Police Forensic Science Laboratory
, London
, 1978
, pp. 2-23-2
-25
.5.
Budowle
, B.
, “Phosphoglucomutase-1 Subtyping of Human Bloodstains on Ultrathin-layer Polyacrylamide Gels
,” Electrophoresis
0173-0835, Vol. 5
, 1984
, pp. 165
-167
.6.
Ornstein
, L.
, “Disk Electrophoresis: I. Background and Theory
,” Annals of the New York Academy of Science
0077-8923, Vol. 121
, 1964
, pp. 321
-349
.7.
Davis
, B.
, “Disk Electrophoresis: II. Method and Application to Human Serum Proteins
,” Annals of the New York Academy of Science
0077-8923, Vol. 121
, 1964
, pp. 404
-427
.8.
Compton
, J.
, McClure
, C.
, and Bonderman
, D.
, “The Use of O-Dianisidine for Serum Haptoglobin Electrophoresis Using Cellulose Acetate
,” Clinical Biochemistry
0009-9120, Vol. 9
, No. 2
, 1976
, pp. 104
-105
.
This content is only available via PDF.
All rights reserved. This material may not be reproduced or copied, in whole or in part, in any printed, mechanical, electronic, film, or other distribution and storage media, without the written consent of ASTM International.
You do not currently have access to this content.