Microgravity (micro-g) environments have been shown to elicit dysregulation of specific genes in a wide assay of cell types. It is known that the activation of transcription factors and molecular signaling pathways influence various physiological outcomes associated with stress and adaptive responses. Nuclear factor-kappa B is one of the most prevailing oxidation-sensitive transcription factors. It is hypothesized that simulated microgravity would activate and its downstream transcriptional networks, thus suggesting a role for in microgravity induced muscle atrophy. To investigate the activation of in a rat cardiac cell line (H9c2) under micro-g, rotating wall vessel bioreactors were used to simulate micro-g conditions. Western blotting revealed that mean nuclear translocation of p65 subunit was 69% for micro-g and 46% for unit-g dynamic control as compared with a 30 min positive control . The results from western blots were confirmed by enzyme-linked immunosorbent assay, which showed 66% for micro-g and 45% for dynamic control as compared with positive control . These results show significant differential translocation of p65 under simulated micro-g. These results may be expanded upon to explain physiological changes such as muscle atrophy and further identify the regulatory pathways and effector molecules activated under exposure to micro-g.
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e-mail: rupak.banerjee@uc.edu
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June 2009
Technical Briefs
Differential Translocation of Nuclear Factor-KappaB in a Cardiac Muscle Cell Line Under Gravitational Changes
Ohwon Kwon,
Ohwon Kwon
Department of Mechanical Engineering,
University of Cincinnati
, Cincinnati, OH 45221
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Michael Tranter,
Michael Tranter
Department of Pharmacology and Cell Biophysics,
University of Cincinnati
, Cincinnati, OH 45221
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W. Keith Jones,
W. Keith Jones
Department of Pharmacology and Cell Biophysics,
University of Cincinnati
, Cincinnati, OH 45221
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John M. Sankovic,
John M. Sankovic
Microgravity Science Division,
NASA Glenn Research Center
, Cleveland, OH 44135
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Rupak K. Banerjee
Rupak K. Banerjee
Department of Mechanical Engineering, and Department of Biomedical Engineering,
e-mail: rupak.banerjee@uc.edu
University of Cincinnati
, Cincinnati, OH 45221
Search for other works by this author on:
Ohwon Kwon
Department of Mechanical Engineering,
University of Cincinnati
, Cincinnati, OH 45221
Michael Tranter
Department of Pharmacology and Cell Biophysics,
University of Cincinnati
, Cincinnati, OH 45221
W. Keith Jones
Department of Pharmacology and Cell Biophysics,
University of Cincinnati
, Cincinnati, OH 45221
John M. Sankovic
Microgravity Science Division,
NASA Glenn Research Center
, Cleveland, OH 44135
Rupak K. Banerjee
Department of Mechanical Engineering, and Department of Biomedical Engineering,
University of Cincinnati
, Cincinnati, OH 45221e-mail: rupak.banerjee@uc.edu
J Biomech Eng. Jun 2009, 131(6): 064503 (4 pages)
Published Online: May 8, 2009
Article history
Received:
February 8, 2009
Revised:
March 20, 2009
Published:
May 8, 2009
Citation
Kwon, O., Tranter, M., Jones, W. K., Sankovic, J. M., and Banerjee, R. K. (May 8, 2009). "Differential Translocation of Nuclear Factor-KappaB in a Cardiac Muscle Cell Line Under Gravitational Changes." ASME. J Biomech Eng. June 2009; 131(6): 064503. https://doi.org/10.1115/1.3128718
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