Solute transport through the bone lacunar-canalicular system is believed to be essential for osteocyte survival and function. We have developed an approach that permits direct measurement of real-time solute movement in intact bones by using fluorescence recovery after phtobleaching (FRAP) technology . The movement of vitally injected fluorescence dye (sodium fluorescein) among individual osteocytic lacunae was visualized in situ and the transport was analyzed by using a two-compartment mathematical model of solute diffusion. Our previous studies  suggested that the matrix surrounding osteocytes is similar to the glycocalyx of endothelial cells as first proposed by Weinbaum et al. (1994) . In the present study, we investigated diffusive transport of five fluorescent tracers of different molecular weights and shapes in the bone lacunar-canalicular system (LCS). Our hypothesis is that the solute transport in lacunar-canalicular system is size and shape dependent, due to the molecular sieving of the pericellular matrix.
- Bioengineering Division
Solute Transport in the Bone Lacunar-Canalicular System is Size and Shape Dependent
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Li, W, Pan, J, & Wang, L. "Solute Transport in the Bone Lacunar-Canalicular System is Size and Shape Dependent." Proceedings of the ASME 2008 Summer Bioengineering Conference. ASME 2008 Summer Bioengineering Conference, Parts A and B. Marco Island, Florida, USA. June 25–29, 2008. pp. 945-946. ASME. https://doi.org/10.1115/SBC2008-192896
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